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Mouse IgE (Immunoglobulin E) ELISA Kit

CAT: 1444-EM10549Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1444-EM10549Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Synonyms
IgE
Cross Reactivity
The kit detected Mouse IgE in the samples and no significant cross-species reactions were found
Target
IgE
Type
Colormetric
Field of Research
Immunology
Detection Method
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgE. You can calculate the concentration of Mouse IgE in the samples by comparing the OD of the samples to the standard curve.
Assay Type
Sandwich
Assay Performance Time
3.25h
Sample Type
Serum, plasma and other biological fluids
Detection Range
125-8000ng/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
75ng/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Mouse

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