Keap1 Rabbit Polyclonal Antibody

• Background :

  This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008], disease:Defects in KEAP1 may be a cause of breast cancer., disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma., domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5., enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane., function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome., PTM:Ubiquitinated and subject to proteasomal degradation., similarity:Contains 1 BACK (BTB/Kelch associated) domain., similarity:Contains 1 BTB (POZ) domain., similarity:Contains 6 Kelch repeats., subcellular location:Shuttles between cytoplasm and nucleus., subunit:Homodimer. Interacts with the N-terminal regulatory domain of NF2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Interacts with PGAM5., tissue specificity:Broadly expressed, with highest levels in skeletal muscle.

• Product Name Alternative :

  KEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19

• Gene Name :

  KEAP1

• Gene ID :

  9817

• Swiss Prot :

  Q14145

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC, ICC/IF, ELISA

• Field of Research :

  Ubiquitin mediated proteolysis

• Purification :

  Affinity purification

• Dilution :

  WB 1:500-1:2000, IHC 1:100-1:300, ICC/IF 1:50-1:200, ELISA 1:10000-1:20000

• Form :

  Liquid

• Buffer :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

• Modification :

  Unmodified

• Molecular Weight :

  70kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype :

  IgG