E2F-2 Rabbit Polyclonal Antibody

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibitsfunction:Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner., PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase., similarity:Belongs to the E2F/DP family., subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP., tissue specificity:Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

E2F2; Transcription factor E2F2; E2F-2

E2F2

1870

Q14209

Human, Mouse

Unconjugated

WB, IHC, ICC/IF, ELISA

Stem cell pathway; Cell_Cycle_G1S; Cell_Cycle_G2M_DNA; Protein_Acetylation

Affinity purification

WB 1:500-1:2000, IHC 1:100-1:300, ICC/IF 1:50-1:200, ELISA 1:20000-1:40000

Liquid

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Unmodified

48kDa

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.