NARF Rabbit Polyclonal Antibody

• Background:

  Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. ItPTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Belongs to the NARF family., subunit:Interacts with LMNA and binds to the farnesylated C-terminal domain., tissue specificity:Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain.

• Product Name Alternative:

  NARF; Nuclear prelamin A recognition factor; Iron-only hydrogenase-like protein 2; IOP2

• Gene Name:

  NARF

• Gene ID:

  26502

• Swiss Prot:

  Q9UHQ1

• Host:

  Rabbit

• Reactivity:

  Human, Rat, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, ELISA

• Purification:

  Affinity purification

• Dilution:

  WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:2000-1:20000

• Form:

  Liquid

• Buffer:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

• Modification:

  Unmodified

• Molecular Weight:

  51kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype:

  IgG