CD3EAP Rabbit Polyclonal Antibody

• Background :

  Caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling., function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation., function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex., miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function., PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes., PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Belongs to the eukaryotic RPA34 RNA polymerase subunit family., subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes., subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity) . Isoform 2 interacts with CD3E., caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling., function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation., function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex., miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function., PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes., PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Belongs to the eukaryotic RPA34 RNA polymerase subunit family., subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes., subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity) . Isoform 2 interacts with CD3E.

• Product Name Alternative :

  CD3EAP; ASE1; CAST; PAF49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49

• Gene Name :

  CD3EAP

• Gene ID :

  10849

• Swiss Prot :

  O15446

• Host :

  Rabbit

• Reactivity :

  Human, Rat, Mouse

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC, ICC/IF, ELISA

• Purification :

  Affinity purification

• Dilution :

  WB 1:500-1:2000, IHC 1:100-1:300, ICC/IF 1:50-1:200, ELISA 1:20000-1:40000

• Form :

  Liquid

• Buffer :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

• Modification :

  Unmodified

• Molecular Weight :

  55kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype :

  IgG