LARG Rabbit Polyclonal Antibody

• Background :

  Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014], disease:A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t (11;11) (q23;23) with MLL., function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) . Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., sequence caution:Contaminating sequence. Potential poly-A sequence., similarity:Contains 1 DH (DBL-homology) domain., similarity:Contains 1 PDZ (DHR) domain., similarity:Contains 1 PH domain., similarity:Contains 1 RGSL (RGS-like) domain., subcellular location:Translocated to the membrane upon stimulation., subunit:Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain with IGF1R beta subunit., tissue specificity:Ubiquitously expressed. Isoform 2 is found in jejunum and testis.

• Product Name Alternative :

  ARHGEF12; KIAA0382; LARG; Rho guanine nucleotide exchange factor 12; Leukemia-associated RhoGEF

• Gene Name :

  ARHGEF12

• Gene ID :

  23365

• Swiss Prot :

  Q9NZN5

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC

• Field of Research :

  Regulation of Actin Dynamics; AMPK

• Purification :

  Affinity purification

• Dilution :

  WB 1:500-1:2000, IHC 1:50-1:300

• Form :

  Liquid

• Buffer :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

• Modification :

  Unmodified

• Molecular Weight :

  173kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype :

  IgG