WAVE1 Rabbit Polyclonal Antibody

• Background:

  The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP) -family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008], domain:Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain., function:Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton., similarity:Belongs to the SCAR/WAVE family., similarity:Contains 1 WH2 domain., subcellular location:Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas., subunit:Component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity) . Binds actin and the Arp2/3 complex. Interacts with BAIAP2., tissue specificity:Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

• Product Name Alternative:

  WASF1; KIAA0269; SCAR1; WAVE1; Wiskott-Aldrich syndrome protein family member 1; WASP family protein member 1; Protein WAVE-1; Verprolin homology domain-containing protein 1

• Gene Name:

  WASF1

• Gene ID:

  8936

• Swiss Prot:

  Q92558

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, ICC/IF, ELISA

• Field of Research:

  Adherens_Junction; Fc gamma R-mediated phagocytosis; Regulates Actin and Cytoskeleton

• Purification:

  Affinity purification

• Dilution:

  WB 1:500-1:2000, IHC 1:100-1:300, ICC/IF 1:200-1:1000, ELISA 1:20000-1:40000

• Form:

  Liquid

• Buffer:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

• Modification:

  Unmodified

• Molecular Weight:

  70kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype:

  IgG