NOS2 Rabbit Polyclonal Antibody

• Background:

  Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008], catalytic activity:L-arginine + n NADPH + n H (+) + m O (2) = citrulline + nitric oxide + n NADP (+) ., cofactor:Binds 1 FAD., cofactor:Binds 1 FMN., cofactor:Heme group., cofactor:Tetrahydrobiopterin (BH4) . May stabilize the dimeric form of the enzyme., enzyme regulation:Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity., function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions., induction:By endotoxins and cytokines., online information:Nitric oxide synthase entry, similarity:Belongs to the NOS family., similarity:Contains 1 FAD-binding FR-type domain., similarity:Contains 1 flavodoxin-like domain., subunit:Homodimer. Binds SLC9A3R1., tissue specificity:Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

• Product Name Alternative:

  NOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

• Gene Name:

  NOS2

• Gene ID:

  4843

• Swiss Prot:

  P35228

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, ICC/IF, ELISA

• Field of Research:

  Arginine and proline metabolism; Calcium; Pathways in cancer; Small cell lung cancer

• Purification:

  Affinity purification

• Dilution:

  WB 1:500-1:2000, IHC 1:50-1:300, ICC/IF 1:50-1:200, ELISA 1:10000-1:20000

• Form:

  Liquid

• Buffer:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

• Modification:

  Unmodified

• Molecular Weight:

  131kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype:

  IgG