Alpha Actinin Rabbit Monoclonal Antibody

• Background:

  Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

• Product Name Alternative:

  BDPLT15

• Gene Name:

  Alpha Actinin

• Gene ID:

  87

• Swiss Prot:

  P12814

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Applications:

  WB, ICC/IF, IP

• Purification:

  Affinity Purification

• Dilution:

  WB 1:500-1:2000, ICC/IF 1:200-1:1000, IP 1:20-1:50

• Form:

  Liquid

• Buffer:

  Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW:103 kDa; Observed MW:103 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Isotype:

  IgG