APPBP1 Rabbit Monoclonal Antibody

• Background:

  Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

• Product Name Alternative:

  NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1

• Gene Name:

  NAE1

• Gene ID:

  8883

• Swiss Prot:

  Q13564

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Applications:

  WB, IP

• Field of Research:

  Cell Biology

• Purification:

  Affinity Purification

• Dilution:

  WB 1:500-1:1000, IP 1:20-1:50

• Form:

  Liquid

• Buffer:

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW: 60 kDa; Observed MW: 60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype:

  IgG