β-Gal Senescence Staining Kit

1.Before use, centrifuge the product to the tube bottom immediately before proceeding with subsequent experiments. 2. The senescence β-galactosidase detection relies on specific pH conditions. Since high CO2 concentrations in CO2 incubators can affect the pH of staining working solution, 37°C incubation should not be performed in CO2 incubators. 3. X-Gal solution may freeze when stored at-20°C °C or 4°C °C. It can be fully thawed by 2-5 minutes at room temperature or in a 37°C water bath with gentle shaking. For aliquoting the solution or preparing staining working solutions, use polypropylene (PP) or glass materials instead of polystyrene (PS) containers such as cell culture plates or serum pipettes. 4. Minor sediment may appear at the bottom of tube B of β-galactosidase staining solution. Shake vigorously to ensure complete dissolution before staining. 5. This kit is theoretically applicable to frozen sections but not paraffin sections. For staining, refer to relevant literature and conduct preliminary experiments to optimize protocols. 6. β-galactosidase staining fixative contains toxic and corrosive substances. For safety, wear lab coats and disposable gloves to avoid direct contact with human skin or inhalation. 7. This product is strictly for research purposes only and must not be used for clinical diagnosis or treatment.