Recombinant Human GM-CSF (C-6His)

• Background:

  Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.

• Product Name Alternative:

  Granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; CSF

• Accession Number:

  P04141

• Host:

  Human Cells

• Endotoxin:

  <1 EU/µg as determined by LAL test.

• Purity:

  Greater than 95% as determined by reducing SDS-PAGE

• Form:

  Lyophilized

• Reconstitution:

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Molecular Weight:

  15.5 KDa

• Shipping Conditions:

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

• Construct:

  Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our Mammalian expression system and the target gene encoding Ala18-Glu144 is expressed with a 6His tag at the C-terminus.

• Species:

  Human