Recombinant Mouse IL-36a

Interleukin-36 alpha (Il-36a) is a member of the IL-1 family.IL¬1α, IL¬1β and IL-18 are potent inflammatory cytokines whose activities are dependent on heterodimeric receptors of the IL-1R superfamily, and which are regulated by soluble antagonists. Il36a is a cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. It is a part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. It seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. It Induces the production of proinflammatory cytokines, including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23 in bone marrow-derived dendritic cells (BMDCs) . Moreover, it is involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II and can induce the production of IFN-gamma, IL-4 and IL-17 by cultured CD4+ T cells and splenocytes. Il36a may play a role in proinflammatory effects in the lung: induces the expression of CXCL1 and CXCL2 in the lung, and the expression of TNF-alpha, IL-36c, IL-1A, IL-1B, CXCL1 and CXCL2 in isolated splenic CD11c+ alveolar macrophages. It may be involved in T cell maturation by stimulating the surface expression of CD40 and modestly CD80 and CD86 in splenic CD11c+ cells and CD4+ T cell proliferation.

Interleukin-36 alpha; Il36a; FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; nterleukin-1 family member 6; IL-1F6; Interleukin-1 homolog 1; IL-1H1; Fil1e; Il1e; Il1f6; Il1h1

Q9JLA2

<1 EU/µg as determined by LAL test.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

17.27 KDa

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Mouse Interleukin-36 Alpha is produced by our E.coli expression system and the target gene encoding Arg8-His160 is expressed.