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Recombinant Mouse Factor X (C-6His)

CAT: 1444-PHM0428-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:1444-PHM0428-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mouse coagulation factor X / F10 a member of the peptidase S1 family. The mature F10 is composed mostly of two EGF-like domains, one Gla gamma-carboxy-glutamate domain and one peptidase S1 domain. Factor Xa is a vitamin K-dependent plasma protease that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. The two chains of F10 are formed from a single-chain precursor by the excision of two Arg residues. A single-chain precursor is initially synthesized in the liver. The light and heavy chains are linked together by disulfide bonds. The light chain contains a Gla and two EGF-like domains. The heavy chain corresponds to the serine protease domain. It can form a heterodimer with SERPINA5.
Product Name Alternative
F10; Coagulation factor X; Stuart factor
Accession Number
O88947
Host
Human Cells
Endotoxin
<1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE
Form
Lyophilized
Reconstitution
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
34.6&18.4 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 1mM CaCl2, pH 7.5.
Construct
Recombinant Mouse CoagulationFactor X is produced by our Mammalian expression system and the target gene encoding Gly21-Asn481 is expressed with a 6His tag at the C-terminus.
Species
Mouse