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Recombinant Rat CD73 (C-6His)

CAT: 1444-PHR1890-01Size: 10 µgDry Ice: YesHazardous: No
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CAT#:1444-PHR1890-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CD73, also known as Ecto-5-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Product Name Alternative
CD73; E5NT; Nucleotidase; NT5E; ecto-5-nucleotidase
Accession Number
P21588
Host
Human Cells
Endotoxin
<1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE
Form
Liquid
Molecular Weight
59 KDa
Shipping Conditions
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Construct
Recombinant Rat 5-Nucleotidase/CD73 is produced by our Mammalian expression system and the target gene encoding Trp29-Lys549 is expressed with a 6His tag at the C-terminus.
Species
Rat

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