Recombinant Human Ezrin

• Background :

  Ezrin is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. The N-terminus of ezrin contains a FERM domain which is further subdivided into three subdomains. The C-terminus contain a ERM domain. As a member of the ERM protein family, Ezrin serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. Ezrin probably involved in connections of major cytoskeletal structures to the plasma membrane. The N-terminal FERM domain strongly binds sodium-hydrogen exchanger regulatory factor (NHERF) proteins (involving long-range allostery) . The C-terminal binds to actin, phosphatidylinositol bis-phosphate (PIP2) and membrane proteins like CD44 and ICAM-2. In epithelial cells, Ezrin is required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, Ezrin is required for normal macropinocytosis.

• Product Name Alternative :

  Ezrin; Cytovillin; Villin-2; p81; EZR; VIL2

• Accession Number :

  P15311

• Host :

  E.coli

• Endotoxin :

  <1 EU/µg as determined by LAL test.

• Purity :

  Greater than 95% as determined by reducing SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  69.4 KDa

• Shipping Conditions :

  The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions :

  Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

• Formulation :

  Supplied as a 0.2 μm filtered solution of 10mM HEPES, pH 7.4.

• Construct :

  Recombinant Human Ezrin is produced by our E.coli expression system and the target gene encoding Met1-Leu586 is expressed.

• Species :

  Human