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Recombinant Human TK1 (C-6His)

CAT: 1444-PHH1641-01Size: 10 µgDry Ice: YesHazardous: No
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CAT#:1444-PHH1641-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Thymidine kinase 1 (TK1) belongs to the thymidine kinase family. It is located in the cytoplasm, and phosphorylated on Ser-13 in mitosis during post-translational modification. Two forms of this protein have been identified in animal cells, one in cytosol TK1 and one in mitochondria TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, while it is very low in resting cells. TK1 acts as a homotetramer, and can transform thymidime to thymidine 5-phosphate with the help of ATP
Product Name Alternative
Thymidine kinase; cytosolic; TK1
Accession Number
P04183
Host
Human Cells
Endotoxin
<1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE
Form
Liquid
Molecular Weight
26.5 KDa
Shipping Conditions
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Formulation
Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 5% Trehalose, 5% Mannitol, 500 mM NaCl, 2 mM EDTA, 10% Glycerol, 1 m MDTT, 0.05% Tween80, pH8.0.
Construct
Recombinant Human Thymidine Kinase, Cytosolic is produced by our Mammalian expression system and the target gene encoding Met1-Asn234 is expressed with a 6His tag at the C-terminus.
Species
Human