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GLS polyclonal antibody

CAT: 0384-BS66209-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS66209-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Isoform 1 and isoform 3 are activated by phosphate. Inhibited by BPTES. BPTES binds between subunits and favors dissociation of the tetramer into dimers.
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
GLS1; KIAA0838; Glutaminase kidney isoform mitochondrial; GLS; K-glutaminase; L-glutamine amidohydrolase
Swiss Prot
O94925
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full length protein of human GLS
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF/ICC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 73 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
GLS pAb detects endogenous levels of GLS protein.
Applications Notes
Western blot analysis of GLS expression in SW620 (A), mouse lung (B) whole cell lysates.
Host or Source
Rabbit

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