Recombinant Mouse EGF

• Product Name Alternative:

  Beta urogastrone, beta-urogastrone, EGF, EGF_HUMAN, Epidermal growth factor, HOMG4, OTTHUMP00000219721, OTTHUMP00000219722, Pro epidermal growth factor, URG, Urogastrone

• UniProt:

  P01132

• Expression Region:

  977-1029

• Host:

  E.Coli

• Tag:

  C-terminal His Tag

• Applications:

  Western Blot, ELISA

• Endotoxin:

  < 1 EU/µg as determined by LAL test.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Measured by its ability to inhibits the proliferation of human epithelial A431 cells.The ED50 for this effect is 2-20 ng/ml (QC verified) .

• Form:

  Lyophilized powder

• Reconstitution:

  Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

• Molecular Weight:

  9-14 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20°C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

• Organism Species:

  Mouse