Mouse APLN (Apelin) ELISA Kit

• Abbreviation:

  APLN

• UniProt:

  Q9R0R4

• Cross Reactivity:

  This kit recognizes Mouse APLN in samples. No significant cross-reactivity or interference between Mouse APLN and analogues was observed

• Target:

  APEL; XNPEP2

• Type:

  Competitive-ELISA

• Applications:

  ELISA

• Field of Research:

  Cancer; Immunology; Microbiology; Signal transduction

• Assay Principle:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse APLN. During the reaction, Mouse APLN in the sample or standard competes with a fixed amount of Mouse APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  125-8000 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  75 pg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Species:

  Mouse

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Mouse APLN concentrations in serum, plasma and other biological fluids.