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NEB Stable Competent E. coli (High Efficiency)

CAT: 0549-C3040HSize: 20 AssaysDry Ice: NoHazardous: No
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CAT#:0549-C3040HSize:20 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NEB Stable Competent E. coli (High Efficiency), C3040H, are chemically competent E. coli cells designed for high-efficiency transformation and cloning of unstable DNA inserts, repetitive sequences and plasmids. The strain is particularly suitable for maintaining difficult constructs and for isolating and propagating retroviral and lentiviral clones. With a transformation efficiency of approximately 1-3 × 10⁹ cfu/µg pUC19 DNA, NEB Stable competent cells combine high transformation performance with reduced recombination of cloned DNA. Key Features
  • High-efficiency chemically competent E. coli.
  • Ideal for unstable inserts and repetitive DNA sequences.
  • Suitable for retroviral and lentiviral plasmid cloning.
  • recA1 mutation helps reduce unwanted DNA recombination.
  • endA1 mutation supports high-quality plasmid DNA preparation.
  • Compatible with NEBuilder HiFi DNA Assembly, Gibson Assembly and ligation reactions.
  • Supplied with optimized NEB 10-beta/Stable Outgrowth Medium.
  • T1 phage resistant and free of animal products.
Applications
NEB Stable Competent E. coli C3040H is recommended for high-efficiency transformation, plasmid cloning, cloning unstable DNA, repeat-element cloning, lentiviral vector cloning, retroviral vector cloning and DNA assembly workflows. It is especially useful when conventional cloning strains have difficulty maintaining constructs containing direct repeats, inverted repeats or other recombination-prone DNA sequences.
FAQ
What is C3040H? => C3040H is the catalog number for the 20 × 50 µL format of NEB Stable Competent E. coli (High Efficiency), a chemically competent cloning strain optimized for unstable and repetitive DNA constructs. What is NEB Stable Competent E. coli used for? => It is used for high-efficiency transformation and for cloning unstable inserts, repetitive DNA sequences, retroviral constructs and lentiviral constructs. What is the transformation efficiency of C3040H? => NEB Stable Competent E. coli has a specified transformation efficiency of approximately 1–3 × 10⁹ cfu/µg. Can C3040H be used for Gibson Assembly? => Yes. NEB lists the strain as compatible with Gibson Assembly, NEBuilder HiFi DNA Assembly and standard ligation reactions. Why are NEB Stable cells useful for unstable plasmids? => The strain contains recA1, which reduces recombination of cloned DNA, helping maintain repetitive and otherwise unstable plasmid constructs.

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