Integrin beta-7 Recombinant Antibody [FIB504] (OAAV00363)

• Gene Name:

  Integrin subunit alpha 4

• Gene Aliases:

  269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49D; IA4; integrin alpha-4; integrin alpha-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha.

• Gene ID:

  3676

• Swiss Prot:

  P15692

• Accession Number:

  NP_000876.3

• Reactivity:

  Human

• Immunogen:

  Alpha-4 integrin.

• Target:

  This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

• Clonality:

  Recombinant Monoclonal

• Clone:

  21.6

• Type:

  Recombinant Monoclonal Antibody

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415) . ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877) .

• Format:

  PBS with 0.02% Proclin 300.

• Reconstitution:

  Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

• Fragment:

  IgG1, kappa

• Specificity:

  This antibody binds to human alpha-4-integrin

• NCBI Gene Symbol:

  ITGA4

• Host or Source:

  Mouse

• Protein Name:

  Integrin alpha-4

• Gene Name URL:

  ITGA4

• Nucleotide Accession Number:

  NM_000885.5