Recombinant Protein L

• Description:

  Protein L is a multi-domain protein found on the surface of Peptostreptococcus magnus, also known as Peptostreptococcus magnus Protein L (PPL) . Unlike Protein A or Protein G, Protein L primarily binds to the kappa light chain of antibodies without interfering with the antigen-binding domain. This unique binding mechanism enables Protein L to interact with a broader range of antibody classes and subtypes, including human IgG, IgM, IgA, and IgD containing kappa light chains, as well as mouse IgG, IgM, rat IgG, Fab fragments, and single-chain variable fragments (scFv), among others.

• Product Name Alternative:

  Protein L, Peptostreptococcus magnus Protein L, PPL

• Expression System:

  E. coli

• Nature:

  Recombinant

• Applications:

  Detection and purification of antibodies and antibody fragments (Fab, ScFv) that contain kappa light chains but not the Fc region.

• Stability:

  Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

• Endotoxin:

  Please contact with the lab for this information.

• Purity:

  >90% as determined by SDS-PAGE.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.

• Reconstitution:

  Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

• Shipping Conditions:

  In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

• Notes:

  For research use only.

• Species:

  Peptostreptococcus magnus