Recombinant Human NTN1/Netrin-1 Protein, C-His (Active)

• Product Name Alternative:

  Netrin-1, NTN1, Epididymis tissue protein Li 131P, NTN1L

• Accession Number:

  O95631

• Expression System:

  Mammalian Cells

• Nature:

  Recombinant

• Applications:

  Bioactivity, ELISA, Immunogen, SDS-PAGE, WB

• Stability:

  Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

• Endotoxin:

  <0.1 EU/μg of the protein by the LAL method.

• Purity:

  >90% as determined by SDS-PAGE.

• Bioactivity:

  Measured by its binding ability in a functional ELISA. When recombinant Rat UNC5H2 is immobilized at 5 μg/mL, recombinant human NTN1/Netrin-1 (Cat #: AHB70501) binds with an apparent Kd <1 nM. Measured in a cell proliferation assay using rat schwannoma cells (RT4‑D6P2T) . The ED50 for this effect is 0.5-2.0 μg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.

• Reconstitution:

  Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

• Shipping Conditions:

  In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

• Notes:

  For research use only.

• Species:

  Homo sapiens (Human)