Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (MALS verified)

Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (MALS verified)

• Background: Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.
• Description: Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (ILR-H525b) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 & Gly 22 - Thr 343 (Accession # P24394-1 & NP_001551.1).
• Specifications: Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag is produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 50.2 kDa & 63.1 kDa. Subunit IL-4 R alpha is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-13 R alpha 1 is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 65-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Met 26 - His 232 & Gly 22 - Thr 343
• Host: HEK 293
• Target: IL-4 R alpha & IL-13 R alpha 1
• Conjugation: Unconjugated
• Tag: C-Fc | C-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) at 5 μg/mL (100 μL/well) can bind Human IL-4, His Tag (Cat. No. IL4-H52H9) with a linear range of 0.6-5 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 50.2 kDa & 63.1 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C