Recombinant Synechocystis sp.Ribonuclease J (rnj)

• Product Name Alternative:

  Rnj; slr0551; Ribonuclease J; RNase J; EC 3.1.-.-

• Abbreviation:

  Recombinant Synechocystis sp. rnj protein

• Gene Name:

  Rnj

• UniProt:

  P54123

• Expression Region:

  1-640aa

• Organism:

  Synechocystis sp. (strain PCC 6803 / Kazusa)

• Target Sequence:

  MAKNTQTQALKILPLGGLHEIGKNTCVFEYDDEILLLDAGLAFPTDDMHGVNVVLPDMTYLRENREKIKGMVVTHGHEDHIGGIAYHLKQFDIPIIYGPRLAMALLRDKLEEAGMLERTNLQTVSPREMVRLGKSFVVEFIRNTHSIADSYCLAIHTPLGVVMHSGDFKIDHTPIDGEFFDLQKVAEYGEKGVLCLLSDSTNAEVPGITPSEASVIPNLDRVFSQAEGRLMVTTFASSVHRVNIILSLAQKHQRKVAVVGRSMLNVIAHARKLGYIKCPDNLFVPLKAARNLPDQQQLILTTGSQGEPLAAMTRISNGEHPQIKIRQGDTVVFSANPIPGNTIAVVNTIDRLMMQGANVIYGKHQGIHVSGHASQEEHKMLLALTRPKFFVPVHGEHRMLVKHSQMAQAQGIPSENIVIVNNGDVIELTGDRIRVAGQVPSGIELVDQAGIVHESTMAERQQMAEDGLVTVAAALSKTGTLLAYPEVHCRGVVMTIQPKLLEELIVRTIENFLTERWSEFTHGSNGSTEVSWNALQKELESSLQRLIKRELQSSPMVLLMLQTDTPIELDQVPQNVSTVSATSATPAPRKKVVLTKTPEPKVKAKPEKKVVTTAEPSAQPVSTTKVYRRSRKRSTTSVSS

• Tag:

  N-terminal 6xHis-SUMO-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  An RNase that has 5'-3' exonuclease and possibly endoonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  An RNase that has 5'-3' exonuclease and possibly endoonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay (By similarity) .

• Molecular Weight:

  86.4 kDa

• References & Citations:

  "A large-scale protein protein interaction analysis in Synechocystis sp. PCC6803." Sato S., Shimoda Y., Muraki A., Kohara M., Nakamura Y., Tabata S. DNA Res. 14:207-216 (2007)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length