Illumination™ Renilla Luciferase Enhanced Assay Kit

• Description:

  GoldBio’s Illumination™RenillaLuciferase Enhanced Assay Kit is specifically designed for the measurement ofRenillaluciferase in transfected cell reporter gene assays. This kit is enhanced with a water soluble coelenterazine that is easier to use and store than other conventional coelenterazine products.Renillaluciferase is a commonly used bioluminescent reporter gene that is used to study gene regulation and function bothin vivoas well asin vitro.Renillaluciferase is a monomeric 36 kDa protein isolated from the sea pansy, Renilla reniformis, which catalyzes coelenterazine with oxygen to produce a blue light (480 nm) . There is no need for any post-translational modifications and the protein may be used as a gene reporter immediately after translation. Our Enhanced Assay Kit includes a special buffer which is designed to yield reliable, linear measurements ofRenillaluciferase activity with maximum sensitivity and minimal autoluminescence. This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional5X Passive Lysis Buffer (GoldBio Catalog # L-745) can be purchased separately.Kit components5X Passive Lysis BufferRenillaLuciferase Assay BufferGoldBio Enhanced Coelenterazine