Keratin 18 (Phospho-Ser33) Antibody

• Background:

  KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

• Description:

  This is a Keratin 18 (Phospho-Ser33) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  CK 18; CK18; CYK18; Cytokeratin endo B; K18

• Gene ID:

  3875

• Swiss Prot:

  P05783

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of serine 33 (A-A-S (p) -V-Y) derived from Human Keratin 18 (CK18) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG