PGK1 (Acetyl-Lys388) Antibody

• Background:

  The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) .

• Description:

  This is a PGK1 (Acetyl-Lys388) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  MGC117307; MGC142128; MGC8947; MIG10; PGKA

• Gene ID:

  5230

• Swiss Prot:

  P00558

• Reactivity:

  Human, Mouse

• Immunogen:

  Peptide sequence around acetylation site of lysine 388 (E-D-K (Acetyl) -V-S) derived from Human PGK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC, IP

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100, IP 1:20-1:50

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  45kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG