CDC25A (Phospho-Tyr59) Antibody

• Background:

  Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

• Description:

  This is a CDC25A (Phospho-Tyr59) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  CDC25M3; MPIP1

• Gene ID:

  993

• Swiss Prot:

  P30304

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of tyrosine 59 (S-D-Y (p) -E-Q) derived from Human CDC25A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  70kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG