P47 phox (Phospho-Ser345) Antibody

• Background:

  NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production) .

• Description:

  This is a p47 phox (Phospho-Ser345) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  NCF1; P47 phox; NCF-47K

• Gene ID:

  653361

• Swiss Prot:

  P14598

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of Serine 345 (P-Q-S (p) -P-G) derived from Human Nuclear p47 phox.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  45kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG