AML1 (Phospho-Ser435) Antibody

• Background:

  CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

• Description:

  This is a AML1 (Phospho-Ser435) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  Acute myeloid leukemia 1 protein; CBFA2; CBFA2; alpha 2 subunit; RUNX1

• Gene ID:

  861

• Swiss Prot:

  Q01196

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of Serine 435 (S-N-S (p) -P-T) derived from Human AML1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  53kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG