BLK (Phospho-Tyr501) Antibody

• Background:

  Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor.

• Description:

  This is a BLK (Phospho-Tyr501) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  P55-BLK; EC 2.7.10.2; kinase Blk; B lymphocyte kinase; Tyrosine-protein kinase BLK

• Gene ID:

  640

• Swiss Prot:

  P51451

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of tyrosine 501 (R-Q-Y (p) -E-L) derived from Human BLK.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  57kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG