Bub3 (Phospho-Tyr207) Antibody

• Background:

  Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

• Description:

  This is a Bub3 (Phospho-Tyr207) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  Mitotic checkpoint protein BUB3; Hbub3; BUB3L; BUB3 budding uninhibited by benzimidazoles 3 homolog; budding uninhibited by benomyl

• Gene ID:

  9184

• Swiss Prot:

  O43684

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y (p) -L-D) derived from Human Bub3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:1000

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  40kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG