C-kit (phospho-Tyr936) Antibody

• Background:

  This is the receptor for stem cell factor (mast cell growth factor) . It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K) .

• Description:

  This is a c-kit (phospho-Tyr936) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  KIT; CD117; SCFR; PBT

• Gene ID:

  3815

• Swiss Prot:

  P10721

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of tyrosine 936 (H-I-Y (p) -S-N) derived from Human c-Kit.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  IHC, WB

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG