PKR (Phospho-Thr446) Antibody

• Background:

  Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

• Description:

  This is a PKR (Phospho-Thr446) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  ADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR

• Gene ID:

  5610

• Swiss Prot:

  P19525

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 446 (K-R-T (p) -R-S) derived from Human PKR.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:2000

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG