Merlin (Phospho-Ser518) Antibody

• Background:

  Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.

• Description:

  This is a Merlin (Phospho-Ser518) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  MERL; NF2; Neurofibromin 2; SCH; Schwannomerlin

• Gene ID:

  4771

• Swiss Prot:

  P35240

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of serine 518 (R-L-S (p) -M-E) derived from Human Merlin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IF

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IF 1:100-1:200

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG