HSP27 (Phospho-Ser78) Antibody

• Background:

  Involved in stress resistance and actin organization.

• Description:

  This is a HSP27 (Phospho-Ser78) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; HSP 27; HSP25

• Gene ID:

  3315

• Swiss Prot:

  P04792

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of serine 78 (A-L-S (p) -R-Q) derived from Human HSP27.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC, IF

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100, IF 1:100-1:200

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG