PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) Antibody

• Background:

  The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

• Description:

  This is a PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  P21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK

• Gene ID:

  5058

• Swiss Prot:

  Q13153

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T (p) -M-V) derived from Human PAK1/PAK2/PAK3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  IHC, IF

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  IHC 1:50-1:100, IF 1:100-1:200

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG