Zap-70 (Phospho-Tyr319) Antibody

• Background:

  Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

• Description:

  This is a Zap-70 (Phospho-Tyr319) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  SRK; STD; TZK; STCD; ZAP-70

• Gene ID:

  7535

• Swiss Prot:

  P43403

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y (p) -S-D) derived from Human Zap-70.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC, IF

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IF 1:100-1:200

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG