Human SPARC protein, His Tag (MALS verified)

• Background:

  This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

• Description:

  Human SPARC protein, His Tag (SPC-H52H3) is expressed from human 293 cells (HEK293) . It contains AA Ala 18 - Ile 303 (Accession # P09486) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 34.6 kDa. The protein migrates as 40-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P09486

• Expression Region:

  Ala 18 - Ile 303

• Host:

  HEK293

• Target:

  SPARC

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  34.6 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Human