VE-Statin/EGFL7

• Description:

  EGF-L7 (Epidermal Growth Factor Like Protein 7, Multiple EGF Like Domains Protein 7, VE Statin) is a multi-domain protein containing two EGF like domains and one EMI domain. It is expressed almost exclusively in endothelial cells and functions to promote normal development of the vascular system, particularly tubulogenesis. EGF-L7 is capable of antagonistic binding to Notch receptors resulting in the inhibition of Notch signaling in HUVEC and neural stem cells. In research models inducing hypoxia and subsequent reoxygenation, (H/R), EGF-L7 can inhibit ICAM-1 expression and enhance the inhibition of NF-kappaB activation. Additionally, EGF-L7 can chemoattract endothelial cells and bind to the extracellular matrix. The overexpression of EGF-L7 is observed in various cancers, and is generally correlated with increased metastasis and a poor prognosis. Recombinant human EGF-L7 is a 27.4 kDa protein containing 251 amino acid residues.

• Synonyms:

  EGFL7; NEU1; ZNEU1; VE-STATIN; RP11-251M1.2, NOTCH4-like protein

• NCBI Gene ID:

  51162

• UniProt:

  Q9UHF1

• Accession Number:

  NP_057299.1

• Accession Number mRNA:

  NM_016215.4

• Chromosomal Location:

  9q34.3

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MYRPGRRVCA VRAHGDPVSE SFVQRVYQPF LTTCDGHRAC STYRTIYRTA YRRSPGLAPA RPRYACCPGW KRTSGLPGAC GAAICQPPCR NGGSCVQPGR CRCPAGWRGD TCQSDVDECS ARRGGCPQRC VNTAGSYWCQ CWEGHSLSAD GTLCVPKGGP PRVAPNPTGV DSAMKEEVQR LQSRVDLLEE KLQLVLAPLH SLASQALEHG LPDPGSLLVH SFQQLGRIDS LSEQISFLEE QLGSCSCKKD S

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (e.g. 0.1% BSA/HSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Data not available.

• Length:

  251

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Molecular Weight:

  27.4 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3months at -20°Cto -80°C.

• Host or Source:

  E. coli