TRAIL R2 / CD262 / TNFRSF10B Protein (His & hFc Tag)

• Description:

  Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.

• Synonyms:

  DR5 Protein; KILLER Protein; Ly98 Protein; MK Protein; Tnfrsf10b Protein; TRAILR2 Protein; TRICK2A Protein; TRICK2B Protein; TRICKB Protein

• Accession Number:

  NP_064671.2

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Label:

  His-/hFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  > 95 % as determined by SDS-PAGE

• Bioactivity:

  Immobilized human TNFSF10 at 10 μg/mL (100 μL/well) can bind mouse TNFRSF10B-Fc. The EC50 of mouse TNFRSF10B-Fc is 0.07-0.17 μg/mL.

• Length:

  373

• Form:

  Lyophilized

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  55 kDa (glycosylated monomer)

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells