TNFSF12 Protein (hFc Tag)

• Description:

  TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.

• Synonyms:

  Tnlg4a Protein; TWEAK Protein

• Accession Number:

  NP_001001513.2

• Reactivity:

  Rat

• Cross Reactivity:

  Rat

• Label:

  HFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  > 90 % as determined by SDS-PAGE

• Bioactivity:

  Immobilized Fc-rat TNFSF12 at 10 μg/mL (100 μL/well) can bind biotinylated human Fc-TNFRSF12A. The EC50 of biotinylated human Fc-TNFRSF12A is 0.12-0.28 μg/mL.

• Length:

  405

• Form:

  Lyophilized

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  49 kDa (glycosylated)

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells