TNF-alpha / TNFA Protein (hFc Tag)

• Description:

  Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

• Synonyms:

  DIF Protein; TNF-alpha Protein; TNFA Protein; TNFSF2 Protein; TNLG1F Protein

• Accession Number:

  NP_000585.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  HFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Bioactivity:

  1. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-5 ng/mL. 2. Immobilized Recombinant Human TNF-alpha / TNFA Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFR1/CD120a Protein, Biotinylated. The EC50 is 1-3 ng/mL.

• Length:

  417

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  46 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells