TIGAR

• Description:

  TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Recombinant human TIGAR expressed in E. coli is a 29.9 kDa protein containing 269 amino-acid residues.

• Synonyms:

  TP53-induced glyclysis and apoptosis regulator

• NCBI Gene ID:

  57103

• UniProt:

  Q9NQ88

• Accession Number:

  NP_065108

• Accession Number mRNA:

  NM_020375

• Chromosomal Location:

  12p13.3

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  ARFALTVVRH GETRFNKEKI IQGQGVDEPL SETGFKQAAA AGIFLNNVKF THAFSSDLMR TKQTMHGILE RSKFCKDMTV KYDSRLRERK YGVVEGKALS ELRAMAKAAR EECPVFTPPG GETLDQVKMR GIDFFEFLCQ LILKEADQKE QFSQGSPSNC LETSLAEIFP LGKNHSSKVN SDSGIPGLAA SVLVVSHGAY MRSLFDYFLT DLKCSLPATL SRSELMSVTP NTGMSLFIIN FEEGREVKPT VQCICMNLQD HLNGLTETR

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Data not available.

• Length:

  269

• Form:

  Lyophilized

• Molecular Weight:

  29.9 kDa

• Host or Source:

  E. coli