TGF-alpha

• Description:

  TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-α induces transformation anchorage independence in cultured cells. Human, murine and rat TGF-α are cross-species reactive. Recombinant human TGF-α is a 50 amino acid polypeptide (5.5 kDa) which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

• Synonyms:

  TGFA; TFGA

• NCBI Gene ID:

  7039

• UniProt:

  P01135

• Accession Number:

  NP_003227.1

• Accession Number mRNA:

  NM_003236

• Chromosomal Location:

  2p13

• Reactivity:

  Human

• Cross Reactivity:

  Chicken, Mouse, Rat, Human, Horse

• Sequence:

  VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

• Length:

  50

• Form:

  Lyophilized

• Molecular Weight:

  5.5 kDa

• Host or Source:

  E. coli