Prokineticin-2

• Description:

  Prokineticin-2 (PK2) is a cysteine-rich secreted protein that is expressed in the testis and in lower levels of the small intestine. PK2 regulates various biological functions including gastrointestinal motility, angiogenesis and circadiam rhythms. It is closely related to EG-VEGF (Prokineticin-1) and binds to two orphan B-protein-coupled receptors termed PK-R1 and PK-R2. Recombinant human Prokineticin-2 is an 8.8 kDa protein consisting of 81 amino acid residues and ten cysteine residues that potentially form five pairs of intra-molecular disulfide bonds.

• Synonyms:

  PROK2

• NCBI Gene ID:

  60675

• UniProt:

  Q9HC23

• Accession Number:

  NP_068754.1

• Accession Number mRNA:

  NM_021935.3

• Chromosomal Location:

  3p13

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Rat, Human

• Sequence:

  AVITGACDKD SQCGGGMCCA VSIWVKSIRI CTPMGKLGDS CHPLTRKVPF FGRRMHHTCP CLPGLACLRT SFNRFICLAQ K

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Data not available.

• Length:

  81

• Form:

  Lyophilized

• Molecular Weight:

  8.8 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 1 month, at 4°C for 6 months and at -20 to -80°C up to December 2019. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source:

  E. coli