Pleiotrophin

• Description:

  Human Pleiotrophin (PTN) also called HARP (Heparin affin-regulatory peptide) is a new member of the heparin-binding neurotrophic factor family. PTN and Midkine are structural homologs, and are highly conserved among species. PTN plays an important role in development processes and promotion of neurite extension. Recombinant Pleiotrophin is a 15.4 kDa protein consisting of 136 amino acid residues.

• Synonyms:

  PTN; HARP; HBNF; HBGF8; NEGF1

• NCBI Gene ID:

  5764

• UniProt:

  P21246

• Accession Number:

  NP_002816.1

• Accession Number mRNA:

  NM_002825

• Chromosomal Location:

  7q33

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Rat, Human, Chicken, Hamster, Bacteria

• Sequence:

  GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD

• Assay Protocol:

  Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Data not available.

• Length:

  136

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  15.4 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source:

  E. coli