NP-1 (alpha-Defensin 1)

• Description:

  Defensins (α and β) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins which include NP-1, NP-2 and NP-3, are distinguished from the β-defensins by the pairing of their three disulfide bonds. In addition to antimicrobial activity, NP-1 exhibits chemotactic activity on dendritic cells. NP-1 is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. NP-1 contains a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human NP-1 is a 3.4 kDa protein containing 30 amino acid residues.

• Synonyms:

  Neutrophil Peptide-1, alpha Defensin-1, HNP-1, Cryptdin

• NCBI Gene ID:

  8829

• UniProt:

  Q15818

• Accession Number:

  NP_002513.2

• Accession Number mRNA:

  NM_002522.3

• Chromosomal Location:

  10p12

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  ACYCRIPACI AGERRYGYCI YQGRLWAFCC

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0-10.0 ng/ml.

• Length:

  30

• Form:

  Lyophilized

• Molecular Weight:

  3.4 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source:

  E. coli